8.10.1 - Free Amino Nitrogen in Wort by Spectrophotometry - Manual Method (IM)
- 23/10/2018
- 590
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- Wort
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- IM
- 2015
The determination of the free amino nitrogen content of wort using colorimetry with ninhydrin. The method gives an estimate of amino acids, ammonia and, in addition, the terminal α-amino nitrogen groups of peptides and proteins. Proline is partially estimated at the wavelength used. The method is not specific for α-amino nitrogen since γ-butyric acid which is present in wort gives a colour reaction with ninhydrin.
