ANALYTICA EBC

• 25/10/2018
• 414
• |
• Microbiological Techniques
• |
• 2011

2.5.2 - Bacteria Ribotyping

In trouble shooting and tracing of contamination routes, identification below the species level is often useful. Ribotyping is a DNA fingerprinting technique enabling the discrimination of bacterial strains within the same species from each other. Species identification can be achieved at the same time, if a relevant ribopattern identification database is available. Descriptors: Bacteria Ribotyping, restriction fragment length polymorphism analysis, restriction enzymes, fingerprints (ribopatterns).

It is necessary to use EBC Microbiology 2.3.3.3

• 25/10/2018
• 532
• |
• Yeast Analysis
• |
• 2011

3.1.1.1 - Haemocytometry

Determination of the yeast cell concentration in stored pitching yeast, fermenting or stored beer. Descriptors: Haemocytometry, counting chamber, counting chambers (Thoma, Malassez, Bürker-Türk, Neubauer), counting area.

• 25/10/2018
• 434
• |
• Yeast Analysis
• |
• 2011

3.1.1.2 - Electronic Counter

Instrumental determination of the yeast cell concentration in fermenting beer or pitching yeast. Descriptors: Electronic Counter, modifies the resistance between the electrodes, electrolyte.

• 25/10/2018
• 502
• |
• Yeast Analysis
• |
• 2011

3.1.1.3 - Photometric Determination

Determination of the yeast cell concentration of stored pitching yeast, fermenting or stored beer with counts over 1 million cells per ml. A concentration step is necessary for samples containing less than 1 million cells. Descriptors: Photometric Determination,  photometer,  calibration curves.

It is necessary to use EBC Microbiology 3.1.1.1 or 3.1.2.2

• 25/10/2018
• 502
• |
• Yeast Analysis
• |
• 2017

3.1.1.4 - Count and Viability of Yeast Cells with a Flow Cytometer

Instrumental determination of yeast cell concentration and yeast viability in brewing yeast containing samples.

• 25/10/2018
• 483
• |
• Yeast Analysis
• |
• 2011

3.1.2.1 - Centrifugation

The rapid estimation of yeast fraction in a slurry. Descriptors: Centrifugation of yeast mass, alkaline treatment.

• 25/10/2018
• 0
• |
• Yeast Analysis
• |
• 2011

3.1.2.1 - Dry Weight

Determination of the concentration of yeast in suspension by means of its dry weight. Descriptors: Dry Weight of Yeast, centrifugation, alkaline solution.

• 25/10/2018
• 437
• |
• Yeast Analysis
• |
• 2011

3.2.1.1 - Methylene Blue/Violet Stain

Rapid estimation of the percentage of “viable” yeast cells by counting dead cells. The method is applicable to all samples containing yeast. Descriptors: Methylene Blue / Violet Stain, ratio between total and dead cells.

• 25/10/2018
• 434
• |
• Yeast Analysis
• |
• 2011

3.2.1.2 - Fluorescence Stain

Rapid estimation of the percentage of “viable” yeast cells by counting dead cells. Descriptors: Fluorescence Stain for yeast analysis,  fluoresce under UV-light, percentage of fluorescing cells.

• 25/10/2018
• 413
• |
• Yeast Analysis
• |
• 2011

3.2.2.1 - Slide Culture Techniques

Estimation of the percentage of viable cells capable of reproducing in a yeast sample. Descriptors: Slide culture techniques for yeast analysis, micro-colonies.

• 25/10/2018
• 519
• |
• Yeast Analysis
• |
• 2011

3.3.1.1 - Cell Morphology

Primary differentiation of yeasts based on cell morphology. Descriptors: cell morphology of yeasts,  size, shape, vegetative reproduction of the cells, examine only pure cultures.

• 25/10/2018
• 422
• |
• Yeast Analysis
• |
• 2011

3.3.2.1 - Yeast Giant Colonies

Quantitative estimate of the proportions of the different brewing yeast strains present in a mixed culture. Descriptors: cell colony morphologie,  yeast giant colonies, long-time incubation.

• 25/10/2018
• 494
• |
• Yeast Analysis
• |
• 2011

3.3.2.2 - Morphology on WLN Agar

The method is intended for breweries that use different yeast strains in fermentation. lt may give a quantitative estimate of the proportions of the different strains present in a mixed culture depending on the strains. Descriptors: yeast morphology on WLN agar.

• 25/10/2018
• 505
• |
• Yeast Analysis
• |
• 2011

3.4.1 - Yeast Storage at Ultra-Low Temperatures

The laboratory storage of brewing yeast stocks cultures over a number of years. It has been demonstrated that the genetic stability and survival of brewing yeast strains are optimal after cryopreservation at ultra-low temperatures (below -139 °C) compared to other preservation methods. The cultures may be stored in plastic tubes or straws. The present method describes the preservation in polypropylene straws under liquid nitrogen or in mechanical freezers. Descriptors: yeast storage at ultra-low temperatures, cryoprotectant agent, long-term storage, cryopreservation,  dehydration, osmotic imbalance, intracellular ice.

• 25/10/2018
• 476
• |
• Yeast Analysis
• |
• 2011

3.4.2 - Yeast subculturing for short-term storage

This method describes the storage of brewing yeast strains for short periods of time (up to 6 months) in the laboratory. Changes in brewing yeast genotype following maintenance of the strains by repeated subculturing have been reported to influence yeast brewing performance. Preservation of the original properties of the stored yeasts in the long term is only recommended at ultra-low temperatures. For short-term storage (up to 6 months), other methods are available that allow safe and simple management of strains in the lab. Repeated subculturing for preservation of brewing yeast should be totally avoided. Descriptors: yeast subculturing for short-term storage.

It is necessary to use EBC Microbology 3.4.1

• 25/10/2018
• 485
• |
• Yeast Analysis
• |
• 2011

3.4.3 - Yeast strain transport

The shipment of brewing yeast strains from a supplier laboratory to a receiving laboratory  where they are to be propagated for industrial purposes. Descriptors: yeast strain transport, short-term storage, free from contaminant microorganisms.

It is necessary to use EBC Microbiology 3.4.2

• 25/10/2018
• 538
• |
• Yeast Analysis
• |
• 2011

3.4.4 - Yeast Propagation (Laboratory Stages)

Propagation of brewing yeast strains from stock cultures to obtain sufficient yeast to pitch the first propagation tank in the brewery. As the objective is to produce large numbers of yeast cells (not to produce beer), the propagation conditions are more flexible than in brewery fermentation, so general guidelines, as well as a standard protocol as a guide are provided. Descriptors: laboratory storage.

it is necessary to use EBC Microbiology 3.4.1 and 3.1

• 25/10/2018
• 480
• |
• Yeast Analysis
• |
• 2011

3.5.1 - Detection and Enumeration of Respiratory Deficient Mutants

The determination of the percentage of cells in a yeast culture which are respiratory deficient (known as “petite mutants”, or simply “petites”). Descriptors: detection and enumeration of respiratory deficient mutants, deficiency in metabolism, low yield of biomass, diacetyl formation.

• 25/10/2018
• 535
• |
• Yeast Analysis
• |
• 2011

3.5.2 - Yeast Characterisation by Fermentation in EBC Tall Tubes

This technique allows the prediction and evaluation of the stability of brewing characteristics in a strain of yeast, and also permits the study of the influence of certain technological parameters on its behaviour in fermenter. It can also be used to assess the characteristics of newly isolated yeast cultures. Descriptors: yeast characterisation by fermentation in EBC Tall Tubes.

• 25/10/2018
• 399
• |
• Yeast Analysis
• |
• 2011

3.5.3.1 - Gilliland Method

Classification of yeast isolates according to their ability to flocculate or form flocs. Descriptors: Gilliland Method,  yeast analyis,  aggregation of yeast cells.

• 25/10/2018
• 424
• |
• Yeast Analysis
• |
• 2011

3.5.3.2 - Hough Method

Classification of yeast isolates based on their ability to flocculate or form flocs and to de-flocculate. Descriptors: Hough Method, yeast analysis, determine the flocculation characteristic,  appearance of yeast suspensions.

It is necessary to use EBC Microbology 3.5.3.1

• 25/10/2018
• 468
• |
• Yeast Analysis
• |
• 2011

3.5.3.3 - Helm Method

Classification of a suspension of yeast based on its rate of flocculation. Descriptors: Helm Method, sedimentation of yeast, calcium sulphate solution, yeast analysis.

• 26/10/2018
• 506
• |
• Detection of Contaminants
• |
• 2011

4.1.1 - Wort Forcing Test

Qualitative detection of microorganisms present and able to grow in wort. Descriptors: wort forcing test, detection of contaminants in wort, haze and CO2 development.

• 26/10/2018
• 502
• |
• Detection of Contaminants
• |
• 2011

4.1.2 - General Aerobic Count

Quantitative detection of aerobic and facultative microorganisms in wort. Descriptors: general aerobic count, aerobic incubation, growth, Plating method, partially selective or non-selective.

It is necessary to use EBC Microbiology 2.3.3.1 or 2.3.3.2 and 2.3.4.2

• 26/10/2018
• 443
• |
• Detection of Contaminants
• |
• 2011

4.1.3.1 - Enterobacteriaceae

Quantitative detection of Enterobacteriaceae in wort. Descriptors: selective media for the growth of Enterobacteriaceae, aerobic incubation.

• 26/10/2018
• 476
• |
• Detection of Contaminants
• |
• 2011

4.1.3.2 - Lactic Acid Bacteria

Quantitative detection of lactic acid bacteria in wort. Descriptors. contaminants, differentiated into rods or cocci, incubation.

It is necessary to use EBC Method 2.3.3.2 and 2.3.4.3 or 2.3.4.4 and 2.3.5.1 or 2.3.5.2 or 2.3.5.4 and 2.3.7 and 2.3.6.1 or 2.3.6.2.

• 26/10/2018
• 460
• |
• Detection of Contaminants
• |
• 2011

4.2.1 - Enrichment

Detection of bacteria in yeast or fermenting beer by the addition of growth medium to the sample. Descriptors: enrichment, concentrated medium, growth of contaminant microorganisms.

• 26/10/2018
• 554
• |
• Detection of Contaminants
• |
• 2011

4.2.2 - General Aerobic Count on Samples of Yeast or Fermenting Beer

Quantitative detection of aerobic and facultative microorganisms in samples where brewer’s yeast is present, for example, yeast slurry from storage vessel, fermentation samples, beer for cask racking, beer in cask, bottled conditioned beer, etc. Descriptors: General Aerobic Count on Samples of Yeast or Fermenting Beer, aerobic incubation, Plating method, cycloheximide, counnt colonies.

it is necessary to use EBC Microbiology 2.3.3.2 and 2.3.4.2

• 26/10/2018
• 443
• |
• Detection of Contaminants
• |
• 2011

4.2.3 - General Anaerobic Count on Samples of Yeast or Fermenting Beer

Descriptors: general anaerobic count on samples of yeast or fermenting beer.

It is necessary to use EBC Microbiology 4.2.4.1 to 4.2.4.3

• 26/10/2018
• 429
• |
• Detection of Contaminants
• |
• 2011

4.2.4.1 - Enterobacteriaceae

Quantitative detection of Enterobacteriaceae in yeast. Descriptors: selective media for the growth of Enterobacteriaceae.